Associate Scientist, Protein Production & Engineering, BioMedicine Design

 Why Patients Need You

Pfizer’s purpose is to deliver breakthroughs that change patients’ lives. Research and Development is at the heart of fulfilling Pfizer’s purpose as we work to translate advanced science and technologies into the therapies and vaccines that matter most. Whether you are in the discovery sciences, ensuring drug safety and efficacy or supporting clinical trials, you will apply cutting edge design and process development capabilities to accelerate and bring the best-in-class medicines to patients around the world.

What You Will Achieve

Pfizer’s BioMedicine Design (BMD) is seeking a highly motivated Cell and Molecular Biologist to join Biotherapeutics Expression and Purification group (BEP) located in Pfizer Worldwide Biotherapeutics Research campus of Cambridge MA. Qualified candidate will play an essential role in support of large-scale non-GMP transient protein lead production across Pfizer’s diverse biotherapeutics discovery portfolio to enable Pfizer’s research units and their pre-clinical therapeutics in-vitro and in-vivo needs. This individual will work in the area of therapeutic protein production and engineering in mammalian cells. The individual will perform mammalian cell culture, molecular biology, large-scale mammalian cell expression, and protein production analysis for supporting protein therapeutics discovery efforts. The individual will perform this function with supervision, toward considerable independence, and will display initiative in learning new techniques. The candidate will contribute to the group wide deliverables and be required to work on several projects simultaneously presenting results and updates at group meetings.

How You Will Achieve It

• Carry out large scale mammalian cell culture, molecular biology, large scale transient transfection, expression analysis with HPLC Protein A capturing, analytical SEC, immunoblotting, Octet analysis, SDS-PAGE analysis, and scale-up production processes.

• Maintain and operate various cell hosts and mutant lines (HEK293 and CHO) for cell culture scale-up, perform downstream mammalian cell culture harvesting and processing.

• Carry out routine plasmid DNA complexation with Tecan Fluent automation system and molecular biology.

• Perform calculations and draws conclusions regarding progresses and results of work.

• Assist with the organization and maintenance of cell line stocks and plasmid DNA stocks.

• Quickly learns and accurately performs new procedures involving a range of technologies.

• Demonstrate oral and written presentation skills.

• Drive technology innovation: design and conduct testing of new process methods, technologies, and products for production analysis.

• Work closely with multidisciplinary teams including therapeutic discovery teams, research units, bioanalytical teams, and R&D project leads.

• Prepare, evaluate, and present data internally to cross functional teams. - Prepare and present project summaries at team meetings, departmental meetings, and through summary reports.

• Contribute to the preparation of papers for peer review journals.

Qualifications

Must Have

• Bachelor’s degree is required with a Life Science major. with 1 year of relevant laboratory experience along with an understanding of biotherapeutics production and between 0 to 3 years of experience

• Mammalian protein expression experience, large-scale HEK293/CHO transient expression experience i

• Training in molecular biology and mammalian cell biology and experimental experience using cell culture techniques.

• Experience with Agilent HPLC, BioRad/AKTA FPLC, immunoblot, and mammalian cell virus testing.

• Understanding of the principles involved in molecular and cellular biology. e.g. PCR, cloning, cell culture techniques.

• Demonstrated capability to conduct complex experiments in parallel.

• Strong organizational and communication skills, written and oral.

  
 

PHYSICAL/MENTAL REQUIREMENTS

• Lifting, sitting, standing, walking, bending

• ability to perform mathematical calculations and ability to perform complex data analysis.

Other Job Details
Work Location Assignment: On Premise

The annual base salary for this position ranges from $54,700.00 to $91,100.00. In addition, this position is eligible for participation in Pfizer’s Global Performance Plan with a bonus target of 7.5% of the base salary. We offer comprehensive and generous benefits and programs to help our colleagues lead healthy lives and to support each of life’s moments. Benefits offered include a 401(k) plan with Pfizer Matching Contributions and an additional Pfizer Retirement Savings Contribution, paid vacation, holiday and personal days, paid caregiver/parental and medical leave, and health benefits to include medical, prescription drug, dental and vision coverage. Learn more at Pfizer Candidate Site – U.S. Benefits | (uscandidates.mypfizerbenefits.com). Pfizer compensation structures and benefit packages are aligned based on the location of hire. The United States salary range provided does not apply to Tampa, FL or any location outside of the United States.

Relocation assistance may be available based on business needs and/or eligibility.

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